Abstract

The effect of various regimes of free-convective heat transfer of a Newtonian incompressible fluid on the polymerase chain reaction in a square cavity is numerically studied. As a mathematical model of the process of free convection, the Oberbeck-Boussinesq approximation is considered. For the polymerase chain reaction, the equations of substance transfer are used, taking into account the physicochemical interactions of the components of the mixture. It was believed that the mixture consists of three components. The results of calculations allow us to estimate the effect of convection flow regimes on the overall duration of the reaction and the concentration field of the components, and also to determine the optimum thermal parameters for PCR.